• Home
  • Browse
    • Current Issue
    • By Issue
    • By Author
    • By Subject
    • Author Index
    • Keyword Index
  • Journal Info
    • About Journal
    • Aims and Scope
    • Editorial Board
    • Publication Ethics
    • Peer Review Process
  • Guide for Authors
  • Submit Manuscript
  • Reviewers
  • Contact Us
 
  • Login
  • Register
Home Articles List Article Information
  • Save Records
  • |
  • Printable Version
  • |
  • Recommend
  • |
  • How to cite Export to
    RIS EndNote BibTeX APA MLA Harvard Vancouver
  • |
  • Share Share
    CiteULike Mendeley Facebook Google LinkedIn Twitter
Archives of Pharmaceutical Sciences Ain Shams University
arrow Articles in Press
arrow Current Issue
Journal Archive
Volume Volume 6 (2022)
Issue Issue 1
Volume Volume 5 (2021)
Volume Volume 4 (2020)
Volume Volume 3 (2019)
Volume Volume 2 (2018)
Volume Volume 1 (2017)
Sobaih, A., Abd El Aziz, L., Nassif, N. (2022). HPLC Method for Determination of Chlorhexidine in Pharmaceutical Formulations alone and in presence of Hexamidine and p-chlorocresol, and in Spiked Human Saliva. Archives of Pharmaceutical Sciences Ain Shams University, 6(1), 45-59. doi: 10.21608/aps.2022.108801.1075
Ahmed E Sobaih; Lobna Abd El Aziz; Nancy Nassif. "HPLC Method for Determination of Chlorhexidine in Pharmaceutical Formulations alone and in presence of Hexamidine and p-chlorocresol, and in Spiked Human Saliva". Archives of Pharmaceutical Sciences Ain Shams University, 6, 1, 2022, 45-59. doi: 10.21608/aps.2022.108801.1075
Sobaih, A., Abd El Aziz, L., Nassif, N. (2022). 'HPLC Method for Determination of Chlorhexidine in Pharmaceutical Formulations alone and in presence of Hexamidine and p-chlorocresol, and in Spiked Human Saliva', Archives of Pharmaceutical Sciences Ain Shams University, 6(1), pp. 45-59. doi: 10.21608/aps.2022.108801.1075
Sobaih, A., Abd El Aziz, L., Nassif, N. HPLC Method for Determination of Chlorhexidine in Pharmaceutical Formulations alone and in presence of Hexamidine and p-chlorocresol, and in Spiked Human Saliva. Archives of Pharmaceutical Sciences Ain Shams University, 2022; 6(1): 45-59. doi: 10.21608/aps.2022.108801.1075

HPLC Method for Determination of Chlorhexidine in Pharmaceutical Formulations alone and in presence of Hexamidine and p-chlorocresol, and in Spiked Human Saliva

Article 5, Volume 6, Issue 1, June 2022, Page 45-59  XML PDF (1.15 MB)
Document Type: Original Article
DOI: 10.21608/aps.2022.108801.1075
Authors
Ahmed E Sobaih email ; Lobna Abd El Aziz; Nancy Nassif
Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Ain Shams University, Abasia, 11566, Cairo, Egypt
Abstract
In the current study, a simple, reliable, and quantitative HPLC analytical method was designed to determine Hexamidine Di isethionate (HEX), Chlorhexidine Di gluconate (CHX) and p-chlorocresol (CSOL) in various dosage forms including mouth washes and intimate douches in addition to chlorhexidine determination in spiked human saliva. HEX, CHX and CSOL were determined in coloured aqueous formulations without any sample pre-treatment or extraction steps. The proposed method showed linearity over a concentration range of 0.10 to 25.00 µg/ml of pure HEX, 2.00 to 30.00 µg/ml of pure CHX, and 0.10 to 30.00 µg/ml of pure CSOL and a detection limit of 0.023, 0.057 & 0.026 for HEX, CHX and CSOL respectively. The recoveries for Cyteal® were 100.43 % ± 1.70, 99.06 % ± 0.69 & 98.74 % ± 1.06 for HEX, CHX and CSOL respectively, whereas, for Hexitol® recovery was 100.79 % ± 1.57 for CHX. Furthermore, the proposed method has been employed to detect CHX in spiked human saliva with a recovery of 101.69 % ± 1.38.
Keywords
HPLC; Hexamidine; Chlorhexidine; p-chlorocresol; COVID-19
Statistics
Article View: 38
PDF Download: 41
Home | Glossary | News | Aims and Scope | Sitemap
Top Top

Journal Management System. Designed by NotionWave.