Molecular Detection of Upper Respiratory Tract Viral Infection with RSV, HPIV & Adenovirus among SARS-CoV2 Negative patients with respiratory illness admitted in tertiary care hospital in Eastern India

Mukherjee, Nivedita; Nayek, Swati; Mondal, Sanjoy; Biswas, Tanusri; Nath, Arghya

doi:10.21608/aps.2023.214314.1124

Molecular Detection of Upper Respiratory Tract Viral Infection with RSV, HPIV & Adenovirus among SARS-CoV2 Negative patients with respiratory illness admitted in tertiary care hospital in Eastern India

Document Type : Original Article

Authors

¹ ICMR-DHR Viral Research &amp; Diagnostic Laboratory, Burdwan Medical College, Burdwan, WB, IN.

² Department of Pathology, Burdwan Medical Colleg.e, Burdwan, WB, IN

³ Department of Orthopaedics, Burdwan Medical College and Hospital, Burdwan, WB, IN.

⁴ Department of Microbiology, Burdwan Medical College.

⁵ ICMR-DHR Viral Research &amp; Diagnostic Laboratory, Department of Microbiology, Burdwan Medical College

10.21608/aps.2023.214314.1124

Abstract

Background: The most prevalent disease-causing agents in humans are respiratory viruses, which have a global influence on morbidity and death. The Adenovirus, respiratory syncytial virus (RSV), and parainfluenza viruses (HPIV) are the dominant respiratory viruses that circulate most often throughout all continents. Vaccines and potent antivirals are not yet available, but progress has been made in understanding their biology and the core problems of the host-parasite interaction. Aims & Objective: To study respiratory viral infections among clinical manifestations among COVID-19 negative patients with pseudo symptoms. Materials & Methods: This cross-sectional hospital-based study conducted on VRDL, Burdwan Medical College where we used real-time PCR for SARS CoV2 screening and viral DNA extraction for Adenovirus screening. Real-time PCR through SyBr green was used to detect RSV, HPIV, and Adenovirus in 120 clinical samples. Beta Actin was used as a test control for DNA compatibility for PCR amplification. Result: 120 patients were studied, with 13 being Adenovirus positive, three RSV positive, and two HPIV positive. 35% were infected with Adenovirus. RSV and HPIV also caused infection, with prevalence rates of 5.83% and 14.17%, respectively. Triple-infection was observed in 1 neonatal patient among 120. Conclusion: Triple infections in RSV, HPIV, Adeno, and neonates were found, but none of the 120 SARS-CoV2 infections were mono-infections. This suggests that respiratory viral infections among peudo-COVID19 signs and symptoms may lead to future pandemic, and further research is needed to confirm this.

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