Optimization of antifungal activity by Bacillus subtilis isolate CCASU 2021-4 using Response Surface Methodology

Document Type : Original Article

Authors

1 Department of Microbiology and Immunology, Faculty of Pharmacy, Ain Shams University, Cairo 11566, Egypt

2 Faculty of Pharmacy, Ain Shams University, Cairo 11566, Egypt

Abstract

Fungal infections represent an enormous load on the public especially with the development of resistance to the most currently used antifungal drugs in practice. In the present work, a bacterial isolate coded A3 was recovered from soil and was shown to express antagonistic activity against Candida (C.) albicans ATCC 10231. This isolate was identified as Bacillus (B.) subtilis subsp. spizizenii isolate CCASU 2021-4 using 16S ribosomal RNA sequencing. D-optimal design from response surface methodology (RSM) was used to optimize the environmental variables affecting antifungal activity of the respective isolate. The optimum conditions were a temperature of 30 °C, a pH of 8 and an inoculum size of 5 x 107 cfu/ml, resulting in an enhancement in the antifungal activity by 1.2 fold. This is the first report, to the best of our knowledge, on an antifungal activity from B. subtilis subsp. spizizenii culture broth against human fungal pathogens along with its optimization through RSM.

Keywords

Archives of Pharmaceutical Sciences Ain Shams University
Volume 5, Issue 1
June 2021
Pages 171-183

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