Elsayed, N., Aboshanab, K., Yassien, M., Aboulwafa, M., Hassouna, N. (2021). Scaling up, Kinetic modeling, and Economic analysis of poly (3-hydroxybutyrate) production by Bacillus cereus isolate CCASU-P83. Archives of Pharmaceutical Sciences Ain Shams University, 5(1), 158-170. doi: 10.21608/aps.2021.76831.1061
Noha S. Elsayed; Khaled M. Aboshanab; Mahmoud A. Yassien; Mohammad M. Aboulwafa; Nadia A. Hassouna. "Scaling up, Kinetic modeling, and Economic analysis of poly (3-hydroxybutyrate) production by Bacillus cereus isolate CCASU-P83". Archives of Pharmaceutical Sciences Ain Shams University, 5, 1, 2021, 158-170. doi: 10.21608/aps.2021.76831.1061
Elsayed, N., Aboshanab, K., Yassien, M., Aboulwafa, M., Hassouna, N. (2021). 'Scaling up, Kinetic modeling, and Economic analysis of poly (3-hydroxybutyrate) production by Bacillus cereus isolate CCASU-P83', Archives of Pharmaceutical Sciences Ain Shams University, 5(1), pp. 158-170. doi: 10.21608/aps.2021.76831.1061
Elsayed, N., Aboshanab, K., Yassien, M., Aboulwafa, M., Hassouna, N. Scaling up, Kinetic modeling, and Economic analysis of poly (3-hydroxybutyrate) production by Bacillus cereus isolate CCASU-P83. Archives of Pharmaceutical Sciences Ain Shams University, 2021; 5(1): 158-170. doi: 10.21608/aps.2021.76831.1061
Scaling up, Kinetic modeling, and Economic analysis of poly (3-hydroxybutyrate) production by Bacillus cereus isolate CCASU-P83
1Department of Microbiology and Immunology, Faculty of Pharmacy, Ain Shams University, Cairo 11566, Egypt
21. Department of Microbiology and Immunology, Faculty of Pharmacy, Ain Shams University 2. Faculty of Pharmacy, King Salman International University, Ras-Sedr, South Sinai, Egypt
Abstract
Background: Polyhydroxyalkanoates (PHA) are environmentally friendly polymers produced by many bacteria under nutrient-limited conditions. However, their commercialization is hindered by the production expenses. The present study aimed at cost-effective and efficient production of poly(3-hydroxybutyrate) (PHB) by Bacillus (B.) cereus isolate CCASU-P83. Methods: Through one factor at time optimization study on shake flask, B. cereus CCASU-P83 produced about 50 % polymer per dry weight after 48 hours incubation time. For better evaluation of the fermentation process, kinetic modeling using the Logistic and Leudking-piret models was applied. Results: A preliminary economic analysis was carried out and leads to a 30.8 % reduction in the total cost. In comparison to the findings obtained on the level of shake flask, scaling up to the bioreactor resulted in producing about 53% PHB per dry weight after only 24 hours incubation. These models concluded that B. cereus produced PHB during the growth phase. Analysis of molecular weight of the produced polymer displayed a 26900 g/mole molecular weight with a polydispersity index (PDI) of 1.1. Conclusion: B. cereus CCASU-P83 is a potential candidate for industrial production of PHB polymer using corn oil in a short incubation period which highly reduced the cost of the production process.
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