Scaling up, Kinetic modeling, and Economic analysis of poly (3-hydroxybutyrate) production by Bacillus cereus isolate CCASU-P83

Elsayed, Noha S.; Aboshanab, Khaled M.; Yassien, Mahmoud A.; Aboulwafa, Mohammad M.; Hassouna, Nadia A.

doi:10.21608/aps.2021.76831.1061

Scaling up, Kinetic modeling, and Economic analysis of poly (3-hydroxybutyrate) production by Bacillus cereus isolate CCASU-P83

Document Type : Original Article

Authors

¹ Department of Microbiology and Immunology, Faculty of Pharmacy, Ain Shams University, Cairo 11566, Egypt

² 1. Department of Microbiology and Immunology, Faculty of Pharmacy, Ain Shams University 2. Faculty of Pharmacy, King Salman International University, Ras-Sedr, South Sinai, Egypt

10.21608/aps.2021.76831.1061

Abstract

Background: Polyhydroxyalkanoates (PHA) are environmentally friendly polymers produced by many bacteria under nutrient-limited conditions. However, their commercialization is hindered by the production expenses. The present study aimed at cost-effective and efficient production of poly(3-hydroxybutyrate) (PHB) by Bacillus (B.) cereus isolate CCASU-P83.
Methods: Through one factor at time optimization study on shake flask, B. cereus CCASU-P83 produced about 50 % polymer per dry weight after 48 hours incubation time. For better evaluation of the fermentation process, kinetic modeling using the Logistic and Leudking-piret models was applied. Results: A preliminary economic analysis was carried out and leads to a 30.8 % reduction in the total cost. In comparison to the findings obtained on the level of shake flask, scaling up to the bioreactor resulted in producing about 53% PHB per dry weight after only 24 hours incubation. These models concluded that B. cereus produced PHB during the growth phase. Analysis of molecular weight of the produced polymer displayed a 26900 g/mole molecular weight with a polydispersity index (PDI) of 1.1.
Conclusion: B. cereus CCASU-P83 is a potential candidate for industrial production of PHB polymer using corn oil in a short incubation period which highly reduced the cost of the production process.

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